Journal: Parasites & Vectors

Article Title: LAG3 constrains anti-parasitic response by effector CD4 + T-cell in early Echinococcus multilocularis -infected mice

doi: 10.1186/s13071-026-07246-y

Figure Lengend Snippet: LAG3 blockade promotes early clearance of E. multilocularis in mouse liver. A Establishment of a LAG3-neutralizing antibody model in vivo. B Representative hematoxylin–eosin staining image (left panel ×40, enlarged ×100 on the right panel) in the liver tissue sections of E. multilocularis -infected mice treated with IgG and anti-LAG3 mAb for 4 weeks. C Percentage and area of different lesion types in the liver of E. multilocularis -infected mice treated with IgG ( n = 7) and anti-LAG3 mAb ( n = 5) for 4 weeks. D Representative Sirius red staining image (left panel ×100, enlarged ×200 on the right panel) in the liver tissue sections of E. multilocularis -infected mice treated with IgG and anti-LAG3 mAb for 4 weeks. E Percentage of different lesion types in the liver of E. multilocularis -infected mice treated with IgG ( n = 7) and anti-LAG3 mAb ( n = 5) for 4 weeks. F Percentage of CD4 + T cells, CD4 + Tn, and CD4 + Teff in the liver and spleen of E. multilocularis -infected mice treated with IgG ( n = 5) and anti-LAG3 mAb ( n = 6) for 4 weeks. G Absolute number of CD4 + T cells, CD4 + Tn, and CD4 + Teff in the liver and spleen of E. multilocularis -infected mice treated with IgG ( n = 5) and anti-LAG3 mAb ( n = 6) for 4 weeks. All data are presented as mean + SD. * P < 0.05, ** P < 0.01, *** P < 0.001, n.s., P > 0.05

Article Snippet: Wild-type C57BL/6 mice were administered 200 μg of anti-LAG3 monoclonal antibody (mAb) (clone C9B7W, BioXCell) or an isotype control (Rat IgG1, κ, BioXCell) 2 days and 1 day prior to E. multilocularis infection via intraperitoneal injection (i.p.), followed by subsequent injections of 200 μg antibody or isotype control every 3 days thereafter.

Techniques: In Vivo, Staining, Infection